AdipoGen Life Sciences

Fc (human):FasL, Soluble (human) (rec.)

Product Code:
 
AG-40B-0132
Product Group:
 
Recombinant Proteins
Antibody Clonality:
 
Enzyme
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Shipping:
 
Blue Ice
Storage:
 
-20°C
1 / 1
Oligomerisation of FasL (h) efficiently triggers Jurkat cell death.Method: Jurkat cells were treated O/N with the indicated concentrations of MegaFasL, Soluble (human) (rec.) (Prod. No. AG-40B-0130), Fc:FasL, Soluble (human) (rec.) (Pr
❮❯

Oligomerisation of FasL (h) efficiently triggers Jurkat cell death.Method: Jurkat cells were treated O/N with the indicated concentrations of MegaFasL, Soluble (human) (rec.) (Prod. No. AG-40B-0130), Fc:FasL, Soluble (human) (rec.) (Pr

No additional charges, what you see is what you pay! *

CodeSizePrice
AG-40B-0132-C01010 ug£240.00
Quantity:
AG-40B-0132-30103 x 10 ug£470.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Switzerland.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • References
  • Related Products
  • Show All

Further Information

Alternate Names/Synonyms:
APO-1L; CD95L; CD178; TNFSF6; Fas Ligand
Biological Activity:
Induces apoptosis of human Jurkat T cells at a concentration of <0.2ng/ml.
Concentration:
0.1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. Human FasL (aa 139-281) is fused at the N-terminus to the Fc portion of human IgG1. Source: HEK 293 cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Binds to human and mouse Fas. Purity: >95% (SDS-PAGE). Fc (human):FasL, Soluble (human) is a high activity construct in which two trimeric FasL are artificially linked via the Fc binding domain of human IgG1. This construct very effectively simulates the natural membrane-assisted aggregation of FasL in vivo. FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. It is involved in cytotoxic T cell mediated apoptosis and in T cell development.
Molecular Weight:
~50kDa (SDS-PAGE)
NCBI, Uniprot Number:
P48023
Package Type:
Plastic Vial
Product Description:
Fc (human):FasL, Soluble (human) is a high activity construct in which two trimeric FasL are artificially linked via the Fc binding domain of human IgG1. This construct very effectively simulates the natural membrane-assisted aggregation of FasL in vivo. FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. It is involved in cytotoxic T cell mediated apoptosis and in T cell development.
Purity:
>95% (SDS-PAGE)
Sequence:
Human FasL (aa 139-281) is fused at the N-terminus to the Fc portion of human IgG1.
Source / Host:
HEK 293 cells
Specificity:
Binds to human and mouse Fas.
TAGs:
Fc
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

References

Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003) | Immune response to intravenous immunoglobulin in patients with Kawasaki disease and MIS-C: Y.P. Zhu, et al.; J. Clin. Invest. ahead of print (2021)