AdipoGen Life Sciences

NAD Synthetase (B. subtilis) (rec.)

Product Code:
 
AG-40T-0109
Product Group:
 
Recombinant Proteins
Antibody Clonality:
 
Enzyme
Regulatory Status:
 
RUO
Shipping:
 
Dry Ice
Storage:
 
-80°C
 

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AG-40T-0109-C05050 ug£357.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
NH(3)-dependent NAD(+) Synthetase; General Stress Protein 38; GSP38; Spore Outgrowth Factor B; EC6.3.1.5
Biological Activity:
~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol NAD per min.
Concentration:
0.5mg/ml (Lot specific)
EClass:
32160000
Endotoxin:
N/A
Form (Short):
liquid
Formulation:
Liquid. In 50mM TRIS-HCl, pH 8, containing 75mM sodium chloride, 5% glycerol and 5mM DTT.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Full-length NAD synthetase (Bacillus subtilis). Source: E. coli. Endotoxin content: N/A. Liquid. In 50mM TRIS-HCl, pH 8, containing 75mM sodium chloride, 5% glycerol and 5mM DTT. Purity: >98% (SDS-PAGE). NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
Molecular Weight:
~30.4kDa
NCBI, Uniprot Number:
P08164
Package Type:
Plastic Vial
Product Description:
NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
Purity:
>98% (SDS-PAGE)
Sequence:
Full-length NAD synthetase (Bacillus subtilis).
Source / Host:
E. coli
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.

References

Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis: M. Rizzi, et al.; EMBO J. 15, 5125 (1996)