NAD Nucleotidase (H. influenza) (rec.)
Product Code:
AG-40T-0110
AG-40T-0110
Antibody Clonality:
Enzyme
Enzyme
Regulatory Status:
RUO
RUO
Shipping:
Dry Ice
Dry Ice
Storage:
-80°C
-80°C
No additional charges, what you see is what you pay! *
| Code | Size | Price |
|---|
| AG-40T-0110-C050 | 50 ug | £357.00 |
Quantity:
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This product comes from: Switzerland.
Typical lead time: 7-10 working days.
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Typical lead time: 7-10 working days.
Contact us for more accurate information.
- Further Information
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Further Information
Alternate Names/Synonyms:
NadN; NAD Pyrophosphatase/5'-nucleotidase
Biological Activity:
With AMP: 856.4 nmol of Ade/min/mg of protein
With NMN: 110,3 nmol of NR/min/mg of protein
With NAD: 5.4 nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein
Concentration:
0.5mg/ml (Lot specific)
EClass:
32160000
Endotoxin:
N/A
Form (Short):
liquid
Formulation:
Liquid. In 5mM KH2PO4, pH 6.8 containing 100mM TRIS-HCl (pH 8.5) and 10mM MgCl2.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Full-length NAD 5?-nucleotidase (Haemophilus influenzae) without the signal peptide corresponding to the first 26 aa. Source: E. coli. Endotoxin content: N/A. Liquid. In 5mM KH2PO4, pH 6.8 containing 100mM TRIS-HCl (pH 8.5) and 10mM MgCl2. Purity: >98% (SDS-PAGE). NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi
Molecular Weight:
~63.0kDa
NCBI, Uniprot Number:
Q4QNY4
Package Type:
Plastic Vial
Product Description:
NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi
Purity:
>98% (SDS-PAGE)
Sequence:
Full-length NAD 5?-nucleotidase (Haemophilus influenzae) without the signal peptide corresponding to the first 26 aa.
Source / Host:
E. coli
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.
Documents
References
The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism: S. Garavaglia, et al.; Biochem. J. 441, 131 (2012)