ProSci

Bcl-G Antibody

Product Code:
 
PSI-3165
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.
2 / 3
Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL.
3 / 3
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 μg/mL.
❮❯

Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.
Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 μg/mL.

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CodeSizePrice
PSI-3165-0.02mg0.02mg£150.00
Quantity:
PSI-3165-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Bcl-G Antibody: BCLG, BCLG, Apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14
Application Note:
Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.
Background References:
  • Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
  • Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
  • Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.
Buffer:
Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen:
Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G.

The immunogen is located within the first 50 amino acids of Bcl-G.
NCBI Gene ID #:
79370
NCBI Official Name:
BCL2-like 14 (apoptosis facilitator)
NCBI Official Symbol:
BCL2L14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NM_030766
Protein GI Number:
13540528
Purification:
Bcl-G Antibody is Ion exchange chromatography purified.
Research Area:
Apoptosis
SPECIFICITY:
Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
Swissprot #:
Q9BZR8
User NOte:
Optimal dilutions for each application to be determined by the researcher.