ProSci

EMX1 Antibody

Product Code:
 
PSI-6625
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Chicken
Antibody Isotype:
 
IgY
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
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Western blot analysis of EMX1 in rat liver tissue lysate with EMX1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of EMX1 in rat liver tissue lysate with EMX1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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CodeSizePrice
PSI-6625-0.02mg0.02mg£150.00
Quantity:
PSI-6625-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
EMX1 Antibody: Homeobox protein EMX1, Empty spiracles homolog 1
Application Note:
EMX1 antibody can be used for detection of EMX1 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Background:
EMX1 Antibody: EMX1 is a homeobox transcription factor involved in specifying cell fates in the developing central nervous system and it participates in the development of olfactory neurons. EMX1 is specifically expressed in the developing telencephalic cortex expression, where epxression is restricted to pyramidal neurons. EMX1 is a reliable marker of pyramidal neurons and pyramidal cell lineage. EMX1 has been shown to be one of the downstream target genes for Gli-Kruppel family member 3 (Gli3) transcription factor, which is a part of the Sonic hedgehog-Patched-Gli signaling pathway important in endocrine signaling.
Background References:
  • Bishop KM, Garel S, Nakagawa Y, et al. Emx1 and Emx2 cooperate to regulate cortical size, lamination, neuronal differentiation, development of cortical efferents, and thalamocortical pathfinding. J. Comp. Neurol. 2003; 457:345-60.
  • Lichtneckert R, Nobs L, Reichert H. Empty spiracles is required for the development of olfactory projection neuron circuitry in Drosophila. Development 2008; 135:2415-24
  • Gulisano M, et al. Emx1 and Emx2 show different patterns of expression during proliferation and differentiation of the developing cerebral cortex in the mouse. Eur. J. Neurosci. 1996; 8:1037-50.
  • Chan CH, Godinho LN, Thomidou D, et al. 2001. Emx1 is a marker for pyramidal neurons of the cerebral cortex. Cereb. Cortex 11:1191-8.
Buffer:
EMX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
EMX1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EMX1.

The immunogen is located within amino acids 50 - 100 of EMX1.
NCBI Gene ID #:
2016
NCBI Official Name:
empty spiracles homeobox 1
NCBI Official Symbol:
EMX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004088
Protein GI Number:
94536800
Purification:
EMX1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least two isoforms of EMX1 are known to exist; this antibody will detect the longer isoform. EMX1 antibody is predicted to not cross-react with other EMX family members.
Swissprot #:
Q04741
User NOte:
Optimal dilutions for each application to be determined by the researcher.