Further Information
EPAC1 Antibody: EPAC, EPAC1, bcm910, HSU79275, CAMP-GEFI, CGEF1, EPAC, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, EPAC 1
EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
EPAC1 Antibody: EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.
- de Rooij J, Zwartkruis FJ, Verheijen MH, et al. Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. Nature 1998; 396:474-7.
- Bos JL. Epac: a new cAMP target and new avenues. Hum. Immunol. 2004; 65:282-90.
- Holz GG, Kang G, Harbeck M, et al. Cell physiology of cAMP sensor Epac. J. Physiol. 2006; 577:5-15.
EPAC1 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
EPAC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EPAC1.
The immunogen is located within amino acids 60 - 110 of EPAC1.
10411
Rap guanine nucleotide exchange factor (GEF) 3
RAPGEF3
Homo sapiens
Liquid
NP_001092001
148747859
EPAC1 Antibody is affinity chromatography purified via peptide column.
Signal Transduction
At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.
O95398
Optimal dilutions for each application to be determined by the researcher.