ProSci

PTCHD2 Antibody

Product Code:
 
PSI-6637
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of PTCHD2 in mouse kidney tissue lysate with PTCHD2 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of PTCHD2 in human kidney tissue with PTCHD2 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of PTCHD2 in human kidney tissue with PTCHD2 antibody at 20 μg/mL.
❮❯

Western blot analysis of PTCHD2 in mouse kidney tissue lysate with PTCHD2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of PTCHD2 in human kidney tissue with PTCHD2 antibody at 2.5 μg/mL.
Immunofluorescence of PTCHD2 in human kidney tissue with PTCHD2 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6637-0.02mg0.02mg£150.00
Quantity:
PSI-6637-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
PTCHD2 Antibody: DISP3, DISP3, KIAA1337, Patched domain-containing protein 2, Protein dispatched homolog 3
Application Note:
PTCHD2 antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PTCHD2 Antibody: PTCHD2, also known as DISP3, is the third of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored secreted proteins. Like DISP1 and DISP2, DISP3 has been implicated in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis. It is highly expressed in Purkinje cells, hippocampal neurons, and retinal ganglion cells. Recently, it has been shown that PTCHD2 localizes within the endoplasmic reticulum and colocalizes with cholesterol, and given that its expression is regulated by thyroid hormone (T3), it has been suggested that DISP3 may be a link between thyroid hormone and cholesterol metabolism.
Background References:
  • Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol. 2005; 26:551-6
  • Katoh Y and Katoh M. Hedgehog signaling pathway and gastric cancer. Can. Biol. & Ther. 2005; 4:1050-4
  • Zikova M, Corlett A, Bendova Z, et al. DISP3, a sterol-sensing domain-containing protein that links thyroid hormone action and cholesterol metabolism. Mol. Endocrin. 2009; 23:520-8.
Buffer:
PTCHD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (89%)
Immunogen:
PTCHD2 antibody was raised against a 19 amino acid synthetic peptide from near the center of human PTCHD2.

The immunogen is located within amino acids 780 - 830 of PTCHD2.
NCBI Gene ID #:
57540
NCBI Official Name:
patched domain containing 2
NCBI Official Symbol:
PTCHD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9P2K9
Protein GI Number:
149274653
Purification:
PTCHD2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least two isoforms of PTCHD2 are known to exist. This antibody is specific for PTCHD2 and will not recognize the other DISP family of proteins.
Swissprot #:
Q9P2K9
User NOte:
Optimal dilutions for each application to be determined by the researcher.