ProSci

CASKIN2 Antibody

Product Code:
 
PSI-6663
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.
2 / 3
Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.
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Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.
Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.
Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6663-0.02mg0.02mg£150.00
Quantity:
PSI-6663-0.1mg0.1mg£449.00
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Further Information

Additional Names:
CASKIN2 Antibody: ANKS5B, KIAA1139, Caskin-2, CASK-interacting protein 2
Application Note:
CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CASKIN2 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting protein ) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN2 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. It is expressed ubiquitously with highest levels present in fetal and adult liver tissue.
Background References:
  • Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13
  • Weng YL, Liu N, DiAntonio A, et al. The cytoplasmic adaptor protein Caskin mediates Lar signal transduction during Drosophila motor axon guidance. Neurosci. 2011; 31:4421-33.
  • Tabuchi K, Biederer T, Butz S, et al. CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with CASKIN2, a novel CASK-binding protein. J. Neurosci. 2002; 22:4264-73.
Buffer:
CASKIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
CASKIN2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human CASKIN2.

The immunogen is located within amino acids 380 - 430 of CASKIN2.
NCBI Gene ID #:
57513
NCBI Official Name:
CASK interacting protein 2
NCBI Official Symbol:
CASKIN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 123, 132 kDa

Observed: 120 kDa
Protein Accession #:
NP_065804
Protein GI Number:
217416347
Purification:
CASKIN2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
CASKIN2 antibody is predicted to not cross-react with other CASKIN family members. Three isoforms of CASKIN2 are known to exist; this antibody will not detect the shortest isoform.
Swissprot #:
Q8WXE0
User NOte:
Optimal dilutions for each application to be determined by the researcher.