ProSci

GRIP1 Antibody

Product Code:
 
PSI-7229
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of GRIP1 in 293 cell lysate with GRIP1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of GRIP1 in rat brain tissue with GRIP1 antibody at 2.5 μg/ml.
3 / 3
Immunofluorescence of GRIP1 in rat brain tissue with GRIP1 antibody at 20 μg/ml.
❮❯

Western blot analysis of GRIP1 in 293 cell lysate with GRIP1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of GRIP1 in rat brain tissue with GRIP1 antibody at 2.5 μg/ml.
Immunofluorescence of GRIP1 in rat brain tissue with GRIP1 antibody at 20 μg/ml.

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CodeSizePrice
PSI-7229-0.02mg0.02mg£150.00
Quantity:
PSI-7229-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
GRIP1 Antibody: GRIP, Glutamate receptor-interacting protein 1, GRIP-1
Application Note:
GRIP1 antibody can be used for detection of GRIP1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
GRIP1 Antibody: Glutamate receptors play an important role in neural plasticity, development and degeneration. The glutamate receptor interacting proteins, GRIP1 and GRIP2, members of the PDZ domain-containing protein family, mediate the trafficking and membrane organization of a number of transmembrane proteins. GRIP1 and GRIP2 specifically bind to the AMPA receptor subunits, GluR 2/3 and are involved in the targeting of GluR 2/3 to the synapse. GRIP1 is expressed in early development before the expression of AMPA receptors, while GRIP2 expression parallels that of AMPA receptors during later developmental stages. GRIP1 and GRIP2 may be involved in the induction of cerebellar long-term depression (LTD).
Background References:
  • Nakanishi S. Molecular diversity of glutamate receptors and implications for brain function. Science 1992; 258:597-603.
  • Dong H, Zhang P, Song I, et al. Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2. J. Neurosci. 1999; 19:6930-41.
  • Mao L, Takamiya K, Thomas G, et al. GRIP1 and 2 regulate activity-dependent AMPA receptor recycling via exocyst complex interactions. Proc. Natl. Acad. Sci. USA 2010; 107:19038-43.
  • Thomas GM, Hayashi T, Chiu SL, et al. Palmitoylation by DHHC5/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking. Neuron 2012; 73:482-96.
Buffer:
GRIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal GRIP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human GRIP1.

The immunogen is located within the last 50 amino acids of GRIP1.
NCBI Gene ID #:
23426
NCBI Official Name:
glutamate receptor interacting protein 1
NCBI Official Symbol:
GRIP1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 118 kDa

Observed: 115 kDa
Protein Accession #:
NP_066973
Protein GI Number:
223890252
Purification:
GRIP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
Q9Y3R0
User NOte:
Optimal dilutions for each application to be determined by the researcher.