ProSci

p53 Antibody

Product Code:
 
PSI-79-146
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p53 (Phospho-Ser15).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser15).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p53 (Phospho-Ser15).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p53 (Phospho-Ser15).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser15).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p53 (Phospho-Ser15).

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PSI-79-146-0.1mg0.1mg£488.00
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Further Information

Additional Names:
P53, BCC7, LFS1, TRP53, MEK6, MKK6, PRKMK6, SKK3, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.
Background References:
  • Lin T, et al. (2005) Nat Cell Biol; 7 (2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23): 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38): 39584-92.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p53 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine 15 (P-L-S (p) -Q-E) derived from Human p53.
Modifications:
Phospho-Specific
NCBI Gene ID #:
7157
NCBI Official Name:
tumor protein p53
NCBI Official Symbol:
TP53
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
NP_000537.3
Protein GI Number:
120407068
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of p53 only whenphosphorylated at serine15.
Swissprot #:
P04637