ProSci

SEK1/MKK4 Antibody

Product Code:
 
PSI-79-186
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using SEK1/MKK4 (Phospho-Ser80).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4 (Phospho-Ser80).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using SEK1/MKK4 (Phospho-Ser80).
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Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using SEK1/MKK4 (Phospho-Ser80).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4 (Phospho-Ser80).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using SEK1/MKK4 (Phospho-Ser80).

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PSI-79-186-0.1mg0.1mg£488.00
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Further Information

Additional Names:
JNKK, MEK4, MKK4, SEK1, SKK1, JNKK1, SERK1, MAPKK4, PRKMK4, SAPKK1, SAPKK-1, SRK, JNKK, MAP2K4, MAPK/ERK kinase 4, MAPKK 4
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Background References:
  • Park HS, et al. (2002) J Biol Chem; 277 (4): 2573-8.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SEK1/MKK4 (Phospho-Ser80) antibody was raised against a peptide sequence around phosphorylation site of serine 80 (T-H-S (p) -I-E) derived from Human SEK1/MKK4.
Modifications:
Phospho-Specific
NCBI Gene ID #:
6416
NCBI Official Name:
mitogen-activated protein kinase kinase 4
NCBI Official Symbol:
MAP2K4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
NP_003001.1
Protein GI Number:
4506889
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of SEK1/MKK4 only when phosphorylated at serine 80.
Swissprot #:
P45985