ProSci

GGA3 Antibody

Product Code:
 
PSI-45-668
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-668 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-668 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-45-668-0.1mg0.1mg£626.00
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Further Information

Additional Names:
GGA3, golgi associated, gamma adaptin ear containing, ARF binding protein 3, HGNC:17079, KIAA0154, ADP-ribosylation factor binding protein 3, Golgi-localized, gamma ear-containing, ARF-binding protein 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 65kDa band in Mouse and Rat Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.0kDa according to NP_766636.2 and 70.2kDa according to NP_001238996.1. The 65kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Background References:
  • Prag G, Lee S, Mattera R, Arighi CN, Beach BM, Bonifacino JS, Hurley JH. Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins. Proc Natl Acad Sci USA. 2005 Feb 15;102(7):2334-9. Epub 2005 Feb 8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EQLSTEVGEVDQ
NCBI Gene ID #:
23163
NCBI Official Name:
golgi-associated, gamma adaptin ear containing, ARF binding protein 3
NCBI Official Symbol:
GGA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_619525.1, NP_054720.1
Protein GI Number:
20336267
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720)
Swissprot #:
Q9NZ52