ProSci

Osteoprotegerin Antibody

Product Code:
 
PSI-XP-5253Bt
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Osteoprotegerin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeat
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To detect hOPG by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human OPG (XP-5253) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hOPG.
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To detect hOPG by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human OPG (XP-5253) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hOPG.

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CodeSizePrice
PSI-XP-5253Bt-0.05mg0.05mg£524.00
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Further Information

Additional Names:
OPG, TR1, OCIF, OPG, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor,
Application Note:
ELISA:

Sandwich:
To detect hOPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human OPG (XP-5253) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG.

Western Blot:

To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOPG (human OPG).
NCBI Gene ID #:
4982
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 11b
NCBI Official Symbol:
TNFRSF11B
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O00300
Protein GI Number:
322510101
Purification:
Anti-hOPG specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Signal Transduction,Antibody Pairs
Swissprot #:
O00300
User NOte:
Centrifuge vial prior to opening.