ProSci

BCA-1 Antibody

Product Code:
 
PSI-XP-5106
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
BCA-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
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To detect hBCA-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human BCA-1 (XP-5106Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBCA-1.
❮❯

To detect hBCA-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human BCA-1 (XP-5106Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBCA-1.

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CodeSizePrice
PSI-XP-5106-0.1mg0.1mg£524.00
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Further Information

Additional Names:
BLC, BCA1, ANGIE, BCA-1, BLR1L, ANGIE2, SCYB13, BLC, C-X-C motif chemokine 13, Angie
Application Note:
ELISA:

To detect hBCA 1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBCA 1.

Western Blot:
To detect hBCA 1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBCA 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
B lymphocyte chemoattractant is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBCA 1 (human B Cell-Attracting Chemokine-1).
NCBI Gene ID #:
10563
NCBI Official Name:
chemokine (C-X-C motif) ligand 13
NCBI Official Symbol:
CXCL13
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O43927
Protein GI Number:
7673945
Purification:
Anti-hBCA 1 specific antibody was purified by affinity chromatography employing immobilized hBCA 1 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
O43927
User NOte:
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.