ProSci

CD3 Monoclonal Antibody

Product Code:
 
PSI-32-130
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
4D10A6
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
CD3 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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FCM analysis CD3 expressed in human peripheral blood cells.
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FCM analysis CD3 expressed in human peripheral blood cells.

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PSI-32-130-0.1mg0.1mg£416.00
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Further Information

Additional Names:
CD3Z, T3Z, TCRZ, T-cell receptor T3 zeta chainT3Z, CD3H, CD3Q, CD3Z, CD3-ZETA
Application Note:
Flow Cytometry and ELISA.
Dilution:
Flow Cytometry :1:200 - 1:400
ELISA:1:10000.
Background:
CD3 is a component of T cell surface receptor (TCR), it cantains 5 invariable chains: gamma- , delta-, epsilon-, and zeta/eta?Clevers et al. (1988) showed the TCR/CD3 complex consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits?CD3 is responsible for the signal transduction of the TCR.
Background References:
  • Clevers,H.Annu.Rev.Immun.6:629-662,1988.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human CD3 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
919
NCBI Official Name:
CD247 molecule
NCBI Official Symbol:
CD247
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P20963
Protein GI Number:
23830999
Purification:
Lyophilized from a 0.2?m filtered solution in PBS.
Research Area:
Immunology
Swissprot #:
P20963
User NOte:
Optimal dilutions for each application to be determined by the researcher.