ProSci

Hdac1 Antibody

Product Code:
 
PSI-43-003
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 2
43-003 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 2

43-003 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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CodeSizePrice
PSI-43-003-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
HDAC1, histone deacetylase 1, RP23-209C6.7, HD1, Hdac1-ps, MommeD5, RPD3, MGC102534, MGC118085, OTTMUSP00000010171
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 55kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1kDa according to Human NP_032254.1 and Mouse NP_032254.1). Recommended concentration: 0.3-1ug/ml.Chromatin Immunoprecipitation: A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PEDAIPEESGDE
NCBI Gene ID #:
3065
NCBI Official Name:
histone deacetylase 1
NCBI Official Symbol:
Hdac1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 55 kDa
Protein Accession #:
NP_032254.1
Protein GI Number:
6680193
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody may cross-react to HDAC2
Swissprot #:
Q13547