ProSci

CHK1 Monoclonal Antibody

Product Code:
 
PSI-32-135
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2G11D5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
CHK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis using CHK1 monoclonal antibody against NIH / 3T3 cell lysate.
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Western blot analysis using CHK1 monoclonal antibody against NIH / 3T3 cell lysate.

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PSI-32-135-0.1mg0.1mg£481.00
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Further Information

Additional Names:
CHK1
Application Note:
Western Blot:Dilution 1:2,00 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Active recombinant full length human CHK1 containing an Nterminal GST tag. This protein has an apparent molecular weight of ~82 kDa. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.
Background References:
  • EMBO J. 1998. 16: 545-554.
  • Science 1998. 282: 1893-1897.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.
NCBI Gene ID #:
1111
NCBI Official Name:
checkpoint kinase 1
NCBI Official Symbol:
CHEK1
NCBI Organism:
Homo sapiens
Protein Accession #:
NP_001107593.1
Protein GI Number:
166295194
Purification:
Antibody is purified by protein G affinity chromatography.
Research Area:
Signal Transduction
Swissprot #:
O14757
User NOte:
Optimal dilutions for each application to be determined by the researcher.