ProSci

C14orf68 Antibody

Product Code:
 
PSI-45-347
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-347 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-347 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-45-347-0.1mg0.1mg£626.00
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Further Information

Additional Names:
C14orf68, chromosome 14 open reading frame 68 , HDMCP, HMFN1655 , hepatocellular carcinoma-downregulated mitochondrial carrier protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml.
Background References:
  • Tan MG, Ooi LL, Aw SE, Hui KM. Cloning and identification of hepatocellular carcinoma down-regulated mitochondrial carrier protein, a novel liver-specific uncoupling protein. J Biol Chem. 2004 Oct 22;279(43):45235-44. Epub 2004 Aug 18.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KSRLQADGQGQRR
NCBI Gene ID #:
283600
NCBI Official Name:
solute carrier family 25, member 47
NCBI Official Symbol:
C14orf68
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_997000.2
Protein GI Number:
46397377
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q6Q0C1