ProSci

p70 S6 Kinase Antibody

Product Code:
 
PSI-79-397
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
1 / 2
Western blot analysis of lysed extracts from 293 cells using p70 S6 Kinase (Ab-424).
2 / 2
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-424).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p70 S6 Kinase (Ab-424).
❮❯

Western blot analysis of lysed extracts from 293 cells using p70 S6 Kinase (Ab-424).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-424).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p70 S6 Kinase (Ab-424).

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-79-397-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
S6K, PS6K, S6K1, STK14A, p70-S6K, p70S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, PKR, PRKR, KS6B1, P70-S6K, RPS6KB1, S6K
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .
Background References:
  • Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24): 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484?97
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p70 S6 Kinase (Ab-424) antibody was raised against a peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.
NCBI Gene ID #:
6198
NCBI Official Name:
ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol:
RPS6KB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70, 85 kDa
Protein Accession #:
NP_003152.1
Protein GI Number:
4506737
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total p70 S6 Kinase protein.
Swissprot #:
P23443