ProSci

PKD/PKCmu Antibody

Product Code:
 
PSI-79-446
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
1 / 2
Western blot analysis of lysed extracts from EGF-treated 293 and PMA-treated HUVEC cells using PKD/PKCμ (Phospho-Ser910).
2 / 2
Immunofluorescence staining of methanol-fixed HeLa cells using PKD/PKCμ (Phospho-Ser910).
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Western blot analysis of lysed extracts from EGF-treated 293 and PMA-treated HUVEC cells using PKD/PKCμ (Phospho-Ser910).
Immunofluorescence staining of methanol-fixed HeLa cells using PKD/PKCμ (Phospho-Ser910).

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PSI-79-446-0.1mg0.1mg£488.00
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Further Information

Additional Names:
PKD, PKCM, PRKCM, PKC-MU, BBC6, BCL2L8, KPCD1, PKC-mu, PKD
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Converts transient diacylglycerol. (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-κ-B.
Background References:
  • Matthews SA, et al. J Biol Chem 1999 Sep; 274 (37): 26543-26549Brandlin I, et al. J Biol Chem 2002 Feb; 277 (8): 6490-6496Storz P, et al. Mol Pharmacol 2004 Oct; 66 (4): 870-879
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PKD/PKCmu (Phospho-Ser910) antibody was raised against a peptide sequence around phosphorylation site of serine 910 (R-V-S (p) -I-L) derived from Human PKD/PKCμ.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5587
NCBI Official Name:
protein kinase D1
NCBI Official Symbol:
PRKD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
115 kDa
Protein Accession #:
NP_002733.2
Protein GI Number:
115529463
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Neuroscience
SPECIFICITY:
This antibody detects endogenous level of PKD/PKCmu only when phosphorylated at serine 910.
Swissprot #:
Q15139