ProSci

PACSIN1 Antibody

Product Code:
 
PSI-45-103
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-103 (1ug/ml) staining of Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-103 (1ug/ml) staining of Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-103-0.1mg0.1mg£626.00
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Further Information

Additional Names:
PACSIN1, protein kinase C and casein kinase substrate in neurons 1, KIAA1379, SDPI, syndapin I
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 51.1kDa according to NP_065855.1). Recommended concentration: 0.03-0.1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex and Cerebellum). Recommended concentration: 2.5ug/ml.
Background References:
  • Modregger J, DiProspero NA, Charles V, Tagle DA, Plomann M. PACSIN 1 interacts with huntingtin and is absent from synaptic varicosities in presymptomatic Huntington's disease brains. Hum Mol Genet. 2002 Oct 1;11(21):2547-58.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: SSSYDEASLAPEET-C
NCBI Gene ID #:
29993
NCBI Official Name:
protein kinase C and casein kinase substrate in neurons 1
NCBI Official Symbol:
PACSIN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065855.1
Protein GI Number:
24308229
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Reported variants represent identical protein (NP_065855.1; NP_001186512.1).
Swissprot #:
Q9BY11