ProSci

NCL Antibody

Product Code:
 
PSI-29-351
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NCL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
1 / 4
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
2 / 4
3 / 4
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.
4 / 4
Antibody used in IHC on Human Skin.
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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.
Antibody used in IHC on Human Skin.

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PSI-29-351-100ul100ul£662.00
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Further Information

Additional Names:
NCL, C23
Application Note:
NCL antibody can be used for detection of NCL by ELISA at 1:500. NCL antibody can be used for detection of NCL by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Background References:
  • Huang, Y., (2006) Blood 107 (9), 3564-3571.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCL.
NCBI Gene ID #:
4691
NCBI Official Name:
nucleolin
NCBI Official Symbol:
NCL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 kDa
Protein Accession #:
NP_005372
Protein GI Number:
55956788
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
RNA Binding
Swissprot #:
P19338
User NOte:
Optimal dilutions for each application to be determined by the researcher.