Further Information
TRIM63, tripartite motif-containing 63 , HGNC:16007, FLJ32380, IRF, MURF1, MURF2, RNF28, SMRZ , iris ring finger protein, muscle specific ring finger protein 1, muscle specific ring finger protein 2, ring finger protein 28, striated muscle RING zinc finger protein
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 75kDa band in Human Heart and Skeletal Muscle lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 1.25ug/ml.
- Cao PR, Kim HJ, Lecker SH. Ubiquitin-protein ligases in muscle wasting. Int J Biochem Cell Biol. 2005 Oct;37(10):2088-97. Epub 2004 Dec 14.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
The immunogen for this antibody is: C-QQYQEQLDKSTKLVE
84676
tripartite motif containing 63, E3 ubiquitin protein ligase
TRIM63
Homo sapiens
Liquid
NP_115977.2
19924163
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
Q969Q1