ProSci

ATF2 Antibody

Product Code:
 
PSI-79-115
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
1 / 2
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53).
2 / 2
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr73 or 55).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Phospho-Thr73 or 55).
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Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr73 or 55).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Phospho-Thr73 or 55).

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PSI-79-115-0.1mg0.1mg£488.00
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Further Information

Additional Names:
HB16, CREB2, TREB7, CREB-2, CRE-BP1, APRF, CREBP1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
Background References:
  • Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33): 12130-12135. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8): 1798-1811. Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATF2 (Phospho-Thr73 or 55) antibody was raised against a peptide sequence around phosphorylation site of threonine 73 or 55 (T-P-T (p) -R-F) derived from Human ATF2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1386
NCBI Official Name:
activating transcription factor 2
NCBI Official Symbol:
ATF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 - 75 kDa
Protein Accession #:
NP_001871.2
Protein GI Number:
22538422
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of ATF-2 only whenphosphorylated at threonine 73 or 55.
Swissprot #:
P15336