ProSci

AGXT Antibody

Product Code:
 
PSI-42-053
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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CodeSizePrice
PSI-42-053-0.1mg0.1mg£505.00
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Further Information

Additional Names:
Agxt, alanine-glyoxylate aminotransferase, Spat, Alanine-glyoxylate aminotransferase (Serine-pyruvate aminotransferase), Serine-pyruvate aminotransferase, angiotensin receptor 2, serine pyruvate aminotransferase precursor (AA -22 to 392), serine:pyruvate aminotransferase SPT, serine:pyruvate/alanine:glyoxylate aminotransferase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx. 22kDa band in Mouse and Rat Liver lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.4kDa according to Rat NP_085914.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Hoshino J, K?hne U, Filjak B, Kröger H. Regulation and characterization of L-serine: pyruvate aminotransferase in rat liver cytosol and mitochondria. Z Naturforsch [C]. 1977 Mar-Apr;32(3-4):249-53
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DKAKSKVYSRKTK
NCBI Gene ID #:
24792 (rat);
NCBI Official Name:
alanine-glyoxylate aminotransferase
NCBI Official Symbol:
Agxt
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
Protein Accession #:
NP_085914.1
Protein GI Number:
13470096
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
Swissprot #:
P09139