ProSci

NEFM Antibody

Product Code:
 
PSI-42-197
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 2
42-197 (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 2
❮❯

42-197 (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-42-197-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
NEFM, neurofilament, medium polypeptide, NEF3, NF-M, NFM, neurofilament triplet M protein, neurofilament, medium polypeptide 150kDa, neurofilament-3 (150 kD medium)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Background References:
  • Levy E, Liem RK, D\1Eustachio P, Cowan NJ Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein European J. Biochem. 1987 Jul 166 (1): 71-7
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QASHITVERKDYLK
NCBI Gene ID #:
4741
NCBI Official Name:
neurofilament, medium polypeptide
NCBI Official Symbol:
NEFM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005373.2
Protein GI Number:
157738649
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell ,Neuroscience
SPECIFICITY:
This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.
Swissprot #:
P07197