Further Information
PRDM1, PR domain containing 1, with ZNF domain, BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1, B-lymphocyte-induced maturation protein 1, OTTHUMP00000016918, PR-domain zinc finger protein 1, PRDI-binding factor-1, beta-interferon gene positive-regulatory domain I binding factor, positive regulatory domain I-binding factor 1
Peptide ELISA: antibody detection limit dilution 1:1000.
Western Blot:Approx 90kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100kDa in lysates of cell line K562. An additional faint band of approx 70kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8kDa according to NP_001189.2 isoform 1 and 76.8kD according to NP_878911.1 isoform 2). Recommended concentration: 0.1-2g/ml. Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
The immunogen for this antibody is: C-DISDNADRLEDVED
639
PR domain containing 1, with ZNF domain
PRDM1
Homo sapiens
Liquid
NP_001189.2, NP_878911.1
172072684
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
O75626