ProSci

SCNN1 gamma Antibody

Product Code:
 
PSI-46-755
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-755 (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-755 (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-46-755-0.1mg0.1mg£626.00
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Further Information

Additional Names:
SCNN1G, sodium channel, nonvoltage-gated 1, gamma, ENaCg, ENaCgamma, PHA1, SCNEG, ENaC gamma subunit, amiloride-sensitive epithelial sodium channel gamma subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 140kDa band in Human Breast cancer and Colon lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.3kDa according to NP_001030.2. The 140kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 4ug/ml.
Background References:
  • Boyd C, Naray-Fejes-T?th A. Steroid-mediated regulation of the epithelial sodium channel subunits in mammary epithelial cells. Endocrinology. 2007 Aug;148(8):3958-67.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SNQLTDTQMLDE
NCBI Gene ID #:
6340
NCBI Official Name:
sodium channel, non-voltage-gated 1, gamma subunit
NCBI Official Symbol:
SCNN1G
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001030.2
Protein GI Number:
42476333
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ,Signal Transduction
Swissprot #:
P51170