ProSci

SF3B3 Antibody

Product Code:
 
PSI-46-363
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-363 staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
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46-363 staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.

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PSI-46-363-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
SF3B3, SAP130, splicing factor 3b, subunit 3, 130kDa, RSE1, SF3B130, KIAA0017, STAF130, pre-mRNA splicing factor SF3b, 130 kDa subunit, spliceosome-associated protein 130, splicing factor 3b, subunit 3, SF3b130
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3ug/ml. Preliminary testing was unsuccessful on NIH3T3 for this particular batch. Primary incubated for 1 hour.
Background References:
  • Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R. Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155. Mol Cell Biol 1999 Oct;19(10):6796-802
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-VSKKLEDIRTRYAF
NCBI Gene ID #:
23450
NCBI Official Name:
splicing factor 3b, subunit 3, 130kDa
NCBI Official Symbol:
SF3B3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036558.3
Protein GI Number:
54112121
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q15393