ProSci

ABCB5 Antibody

Product Code:
 
PSI-46-620
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-620 (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-620 (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-46-620-0.1mg0.1mg£626.00
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Further Information

Additional Names:
ATP-binding cassette, sub-family B (MDR/TAP), member 5 , ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Background References:
  • Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10):4320-33.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QTQHRNTSKKAQ
NCBI Gene ID #:
340273
NCBI Official Name:
ATP-binding cassette, sub-family B (MDR/TAP), member 5
NCBI Official Symbol:
ABCB5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_848654.3, NP_001157413.1
Protein GI Number:
148612844
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Signal Transduction
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)
Swissprot #:
Q2M3G0