ProSci

LEF1 Antibody

Product Code:
 
PSI-27-252
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store LEF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
1 / 1
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

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PSI-27-252-100ul100ul£662.00
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Further Information

Additional Names:
LEF1, DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Application Note:
LEF1 antibody can be used for detection of LEF1 by ELISA at 1:312500. LEF1 antibody can be used for detection of LEF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).
Background References:
  • Petropoulos, K., (2008) J. Exp. Med. 205 (3), 515-522.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1.
NCBI Gene ID #:
51176
NCBI Official Name:
lymphoid enhancer-binding factor 1
NCBI Official Symbol:
LEF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
NP_057353
Protein GI Number:
7705917
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cancer ,Signal Transduction
Swissprot #:
Q9UJU2
User NOte:
Optimal dilutions for each application to be determined by the researcher.