ProSci

Rabenosyn 5 Antibody

Product Code:
 
PSI-46-259
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-259 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-259 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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CodeSizePrice
PSI-46-259-0.1mg0.1mg£626.00
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Further Information

Additional Names:
ZFYVE20, Rabenosyn 5, FYVE-finger-containing Rab5 effector protein rabenosyn-5, zinc finger, FYVE domain containing 20, zinc finger, FYVE domain containing 20, FLJ34993, MGC126210, Rabenosyn-5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration: 0.3 - 1.5 ug/ml.
Background References:
  • Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol. 2000 Oct 30;151(3):601-12.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ELKHTLAKQKGGTD
NCBI Gene ID #:
64145
NCBI Official Name:
zinc finger, FYVE domain containing 20
NCBI Official Symbol:
ZFYVE20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071735.2
Protein GI Number:
70980547
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9H1K0