ProSci

Stearoyl-CoA desaturase Antibody

Product Code:
 
PSI-46-443
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-443 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-443 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-46-443-0.1mg0.1mg£626.00
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Further Information

Additional Names:
SCD, SCD1, HGNC:10571, FADS5, PRO0998 , acyl-CoA desaturase, delta-9-desaturase, fatty acid desaturase, predicted protein of HQ0998, stearoyl-CoA desaturase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Liver and Colon. Recommended concentration: 5ug/ml.Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
Background References:
  • Zhang S, Yang Y, Shi Y. Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells. Biochem J. 2004 Dec 20;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RIKRTGDGNYKSG
NCBI Gene ID #:
6319
NCBI Official Name:
stearoyl-CoA desaturase (delta-9-desaturase)
NCBI Official Symbol:
SCD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005054.3
Protein GI Number:
53759151
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O00767