ProSci

ATG5 Recombinant Protein

Product Code:
 
PSI-95-121
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Mass Spectrometry
  • Neutralisation
  • Western Blot (WB)
Storage:
 
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
 

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CodeSizePrice
PSI-95-121-0.05mg0.05mg£347.00
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Further Information

Additional Names:
ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Background:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy (4). This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells (5).
Background References:
  • Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene . 2004; 23:2891-906.
  • Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
  • Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
  • Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 2007; 282:37298-302.
Buffer:
1X PBS containing 10% glycerol
Fusion Tag:
Fusion Partner: C-terminal His-tag
NCBI Gene ID #:
9474
NCBI Official Name:
ATG5 autophagy related 5 homolog (S. cerevisiae)
NCBI Official Symbol:
ATG5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
34 kDa (Calculated)
Protein Accession #:
AAH02166
Protein GI Number:
10285391
Purity:
~95%
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
A9UGY9

Documents