ProSci

PUMA Fragment Recombinant Protein

Product Code:
 
PSI-95-102
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Mass Spectrometry
  • Western Blot (WB)
Storage:
 
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
 

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PSI-95-102-0.05mg0.05mg£347.00
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Further Information

Additional Names:
PUMA Antibody : JFY1, PUMA, JFY-1
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Background:
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA- (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. This PUMA recombinant protein is recognized by several ProSci PUMA monoclonal antibodies, but not the two polyclonal antibodies (PSI # 3041 and 3043).
Background References:
  • Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
  • Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
  • Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.
Buffer:
1X PBS containing 0.1% SDS and 0.02% NaN3
Fusion Tag:
Fusion Partner: His-tag and strepII-tag at N-terminus
NCBI Gene ID #:
27113
NCBI Official Name:
BCL2 binding component 3
NCBI Official Symbol:
BBC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
15 kDa (Calculated)
Protein Accession #:
Q9BXH1
Protein GI Number:
56748610
Purity:
~95%
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q9BXH1

Documents