ProSci

HRP Rabbit Anti-Chicken IgY Antibody

Product Code:
 
PSI-XR-9120
Product Group:
 
Secondary Antibody
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgY
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Chicken (Gallus)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Peroxidase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not complete
 

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CodeSizePrice
PSI-XR-9120-1.5mg1.5mg£705.00
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Further Information

Application Note:
Peroxidase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:35,000 - 1:150,000, Western Blot 1:3,500 - 1:15,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user).
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Conjugate:
HRP
Immunogen:
Chicken IgY - whole molecule
Physical State:
Lyophilized
Purification:
Peroxidase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgY and Chicken Serum.

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