ProSci

Vimentin (phospho Ser56) Antibody

Product Code:
 
PSI-79-990
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store antibody at -20°C for up to one year.
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Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) Antibody. The lane on the right is treated with the antigen-specific peptide.

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PSI-79-990-0.1mg0.1mg£488.00
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Further Information

Additional Names:
VIME, vimentin
Application Note:
Western Blot: 1:500~1:1000
Background:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Background References:
  • Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Vimentin (phospho Ser56) antibody was raised against a peptide sequence around phosphorylation site of serine 56 (A-S-S (p)-P-G) derived from Human Vimentin.
Modifications:
Phospho-Specific
NCBI Gene ID #:
7431
NCBI Official Name:
vimentin
NCBI Official Symbol:
VIM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
NP_003371.2
Protein GI Number:
62414289
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56.
Swissprot #:
P08670