ProSci

SIRP alpha Recombinant Protein

Product Code:
 
PSI-96-693
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-693-0.1mg0.1mg£741.00
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Further Information

Additional Names:
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Background References:
  • Timms J.F., et al., 1999, Curr. Biol. 9:927-930.
  • Stofega M.R., et al., 2000, J. Biol. Chem. 275:28222-28229.
  • Latour S., et al., 2001, J. Immunol. 167:2547-2554.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CD47, Fc Tag at 2 μg/ml (100 μL/well) can bind Human SIRP alpha, His Tag with a linear range of 16-250 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
140885
NCBI Official Name:
signal-regulatory protein alpha
NCBI Official Symbol:
SIRPA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
38.1 kDa
Protein Accession #:
NP_001035111
Purity:
>95% as determined by SDS-PAGE and SEC-HPLC.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P78324

Documents