Further Information
SerpinA1, PI, A1A, AAT, PI1, A1AT, MGC9222, PRO2275, MGC23330, alpha1AT, SPAAT
This recombinant protein can be used for WB. For research use only.
Serpin A1 is also known as Alpha-1-antitrypsin (A1AT), serum trypsin inhibitor, alpha-1 proteinase inhibitor (A1PI), AAT, which belongs to the serpin family. Most serpins inactivate enzymes by binding to them covalently, requiring very high levels to perform their function. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Serpin A1 / A1AT is inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of SerpinA1 inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Serpin A1 / A1AT protects tissues from enzymes of inflammatory cells, especially neutrophil elastase. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD).
- Tanaka N., et al., 1991, Jpn. J. Cancer Res. 82:693-700.
- Niemann M.A., et al., 1992, Matrix 12:233-241.
- Gettins PG., 2002, Chem Rev 102 (12): 4751?804.
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in 100μl reaction mixture containing 1. 25 ng trypsin, 10μM substrate, 50 mM Tris, 10 mM CaCl2, 0. 15 M NaCl , pH 7. 5. The IC50 value is approximately <4. 5 nM.
PBS, pH7.4
His Tag
5265
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
SERPINA1
Homo sapiens
Lyophilized
Predicted Molecular Weight:
45.1 kDa
AAH11991
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
HEK293 cells
P01009
