ProSci

Erythropoietin Recombinant Protein

Product Code:
 
PSI-96-277
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-277-0.02mg0.02mg£524.00
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Further Information

Additional Names:
EPO, EP, MVCD2, Erythropoietin, Erythropoetin, Erthropoyetin, Hematopoietin, Hemopoietin
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Human Erythropoietin (EPO) is also known as EP, erythropoetin or erthropoyetin, and is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. EPO is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. is synthesized by renal peritubular cells in adults, with a small amount being produced in the liver. Regulation is believed to rely on a feed-back mechanism measuring blood oxygenation. Constitutively synthesized transcription factors for EPO, known as hypoxia-inducible factors (HIFs), are hydroxylated and proteosomally digested in the presence of oxygen. It binds to the erythropoietin receptor (EpoR) on the red cell surface and activates a JAK2 cascade. Erythropoietin has its primary effect on red blood cells by promoting red blood cell survival through protecting these cells from apoptosis. It also cooperates with various growth factors involved in the development of precursor red cells. EPO has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. It has also been shown that erythropoietin can increase iron absorption by suppressing the hormone hepcidin. Erythropoietin has been shown to interact with the Erythropoietin receptor as its mechanism of action within the body. erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.
Background References:
  • Jacobson LO, et al., 1957, Nature 179 (4560): 633?4.
  • Fisher JW, et al., 1996, British journal of haematology 95 (1): 27?32.
  • Jelkmann W ., 2007, Eur. J. Haematol. 78 (3): 183?205.
  • Ashby DR, et al., 2010, Haematologica 95 (3): 505?8.
Biological Activity:
The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0. 2ng/mL, corresponding to a specific activity of >5000000 Unit/mg.
Buffer:
PBS, pH7.4
Fusion Tag:
Tag free
NCBI Gene ID #:
2056
NCBI Official Name:
erythropoietin
NCBI Official Symbol:
EPO
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
18.4 kDa
Protein Accession #:
AAH93628.1
Purity:
>97% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P01588

Documents