ProSci

Flt3 Ligand Recombinant Protein (HEK293 Expressed)

Product Code:
 
PSI-96-321
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-321-0.02mg0.02mg£524.00
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Further Information

Additional Names:
FLT3LG, FL, FLT3L, Flt3 ligand
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.
Background References:
  • Wodnar-Filipowicz A., 2003, News Physiol Sci., 18:247-51.
  • McClanahan T., et al., 1996, Blood, 88(9):3371-82.
  • Diener K.R. et al., 2008, Exp Hematol., 36(1):51-60.
  • Farag SS, Caligiuri MA, 2006, Blood Rev. 20(3):123-37.
Biological Activity:
The bio-activity was determined by dose-dependent stimulation of the proliferation of human acute myeloid leukemia cell line OCI-AML5. The ED50 < 0. 5 ng/mL, corresponding to a specific activity of >2000000 Unit/mg.
Buffer:
PBS, pH7.4
Fusion Tag:
Tag free
NCBI Gene ID #:
2323
NCBI Official Name:
fms-related tyrosine kinase 3 ligand
NCBI Official Symbol:
FLT3LG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
18.9 kDa
Protein Accession #:
AAI26294
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P49771

Documents