ProSci

LIF Recombinant Protein (HEK293 Expressed)

Product Code:
 
PSI-96-519
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-519-0.02mg0.02mg£469.00
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Further Information

Additional Names:
LIF, CDF, DIA, HILDA, MLPLI
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Background References:
  • Wenwei Hu, Zhaohui Feng, Angelika K. Teresky1, Arnold J. Levine (November 29, 2007).
  • Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L (2009). PLoS ONE 4 (7): e6343.
  • Patterson PH (1994). Proc. Natl. Acad. Sci. U.S.A. 91 (17).
Biological Activity:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0. 1 ng/mL, corresponding to a specific activity of >1X107 Unit/mg.
Buffer:
PBS, pH7.4
Fusion Tag:
Tag free
NCBI Gene ID #:
3976
NCBI Official Name:
leukemia inhibitory factor
NCBI Official Symbol:
LIF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
19.9 kDa
Protein Accession #:
AAH69540.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P15018

Documents