ProSci

SCF Recombinant Protein

Product Code:
 
PSI-96-676
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-676-0.02mg0.02mg£524.00
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Further Information

Additional Names:
KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.
Background References:
  • Lu H.S., et al., 1992, Arch. Biochem. Biophys. 298:150-158.
  • Wang Z.-Q., et al., 2009, Am. J. Hum. Genet. 84:672-677.
  • Lev S., et al., 1992, J. Biol. Chem. (UNITED STATES) 267 (15): 10866?73.
Biological Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0. 5 - 8 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
17311
NCBI Official Name:
kit ligand
NCBI Official Symbol:
Kitl
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
19.1 kDa
Protein Accession #:
NP_038626
Purity:
>98% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P20826

Documents