ProSci

VEGF 120 Recombinant Protein (HEK293 Expressed)

Product Code:
 
PSI-96-767
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-767-0.02mg0.02mg£524.00
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Further Information

Additional Names:
VEGF120, VEGFA, VPF
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.
Background References:
  • Shin S.-Y., et al., 2005, Yonsei Med. J. 46:679-686.
  • Breier G., et al., 1992, Development 114:521-532.
Biological Activity:
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
Tag free
NCBI Gene ID #:
22339
NCBI Official Name:
vascular endothelial growth factor A
NCBI Official Symbol:
Vegfa
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
17.2 kDa
Protein Accession #:
AAB22254.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q00731

Documents