ProSci

NIPBL Antibody

Product Code:
 
PSI-43-145
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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43-145 (2ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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43-145 (2ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

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PSI-43-145-0.1mg0.1mg£626.00
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Further Information

Additional Names:
NIPBL, Nipped-B homolog (Drosophila), CDLS, CDLS1, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3, IDN3-B, Scc2, SCC2 homolog, delangin, nipped-B-like, sister chromatid cohesion 2 homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Liu J, Zhang Z, Bando M, Itoh T, Deardorff MA, Clark D, Kaur M, Tandy S, Kondoh T, Rappaport E, Spinner NB, Vega H, Jackson LG, Shirahige K, Krantz ID, Transcriptional dysregulation in NIPBL and cohesin mutant human cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RPDSPRVKQGDSNK
NCBI Gene ID #:
25836
NCBI Official Name:
Nipped-B homolog (Drosophila)?
NCBI Official Symbol:
NIPBL
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
Protein Accession #:
NP_597677.2, NP_056199.2
Protein GI Number:
47578105
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity, Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_597677.2; NP_056199.2).
Swissprot #:
Q6KC79