ProSci

POLRMT Antibody

Product Code:
 
PSI-43-475
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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PSI-43-475-0.1mg0.1mg£505.00
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Further Information

Additional Names:
POLRMT, polymerase (RNA) mitochondrial (DNA directed), APOLMT, MTRNAP, MTRPOL, h-mtRPOL, DNA-directed RNA polymerase, mitochondrial
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave bands at approx 90kDa and 35kDa in Human HepG2 lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 139kDa according to NP_005026.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background:
This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.
Background References:
  • Ringel R, Sologub M, Morozov YI, Litonin D, Cramer P, Temiakov D.Structure of human mitochondrial RNA polymerase.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-KETLQAVPKPGAFD
NCBI Gene ID #:
5442
NCBI Official Name:
polymerase (RNA) mitochondrial (DNA directed)
NCBI Official Symbol:
POLRMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005026.3
Protein GI Number:
110618253
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
Swissprot #:
O00411
User Note:
Optimal dilutions for each application to be determined by the researcher.