ProSci

Lysine--tRNA Ligase Recombinant Protein

Product Code:
 
PSI-91-664
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Storage:
 
Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
 

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PSI-91-664-0.05mg0.05mg£883.00
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Further Information

Additional Names:
Lysine--tRNA Ligase, Lysyl-tRNA Synthetase, LysRS, KARS, KIAA0070
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).
Buffer:
Supplied as a 0.2 um filtered solution of 20mM TrisHCl,150mM NaCl,1mM DTT,20% glycerol,pH8.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Fusion Tag:
C-6 His tag
NCBI Gene ID #:
3735
NCBI Official Name:
lysyl-tRNA synthetase
NCBI Official Symbol:
KARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
69.1 kD
Protein Accession #:
Q15046
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
Human Cells
Swissprot #:
Q15046

Documents