ProSci

MAGEC1 Antibody

Product Code:
 
PSI-62-156
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of MAGEC1 antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of MAGEC1 antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-62-156-400ul400ul£626.00
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Further Information

Additional Names:
Melanoma-associated antigen C1, Cancer/testis antigen 71, CT71, MAGE-C1 antigen, MAGEC1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
MAGEC1 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This protein contains a large number of unique short repetitive sequences in front of the MAGE-homologous sequence, thus is about 800 residues longer than the other MAGE proteins. This gene is expressed in a significant proportion of tumors of various histological types, whereas it is silent in normal tissues, except testis. The function of this gene is unknown.
Background References:
  • Jungbluth, A.A., et al., Int. J. Cancer 99(6):839-845 (2002).
  • Chen, Y.T., et al., Proc. Natl. Acad. Sci. U.S.A. 95(12):6919-6923 (1998).
  • Lucas, S., et al., Cancer Res. 58(4):743-752 (1998).
  • Lim, S.H., et al., Eur. J. Haematol. 67 (5-6), 332-334 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAGEC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 849-880 amino acids from the C-terminal region of human MAGEC1.
NCBI Gene ID #:
9947
NCBI Official Name:
Melanoma-associated antigen C1
NCBI Official Symbol:
MAGEC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
124 kDa
Protein Accession #:
O60732
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
O60732
User NOte:
Optimal dilutions for each application to be determined by the researcher.