ProSci

EphB2 Antibody

Product Code:
 
PSI-63-057
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-EphB2 C-term Pab in NCI-H460 cell lysate
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Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Confocal immunofluorescent analysis of EphB2 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of anti-EphB2 C-term Pab in NCI-H460 cell lysate
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of EphB2 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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PSI-63-057-400ul400ul£626.00
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Further Information

Additional Names:
Ephrin type-B receptor 2, Developmentally-regulated Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase 3, EPH-like kinase 5, EK5, hEK5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3, EPHB2, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.
Background References:
  • Thanos, C.D., et al., Science 283(5403):833-836 (1999).
  • Tang, X.X., et al., Oncogene 17(4):521-526 (1998).
  • Fox, G.M., et al., Oncogene 10(5):897-905 (1995).
  • Ikegaki, N., et al., Hum. Mol. Genet. 4(11):2033-2045 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1021-1050 amino acids from the C-terminal region of human EphB2.
NCBI Gene ID #:
2048
NCBI Official Name:
Ephrin type-B receptor 2
NCBI Official Symbol:
EPHB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
117 kDa
Protein Accession #:
P29323
Protein GI Number:
76803654
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P29323
User NOte:
Optimal dilutions for each application to be determined by the researcher.