ProSci

ARNT2 Antibody

Product Code:
 
PSI-58-559
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in 293 cell line lysates (35ug/lane).
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Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody . SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.
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Western blot analysis in 293 cell line lysates (35ug/lane).
Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody . SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.

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PSI-58-559-400ul400ul£626.00
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Further Information

Additional Names:
Aryl hydrocarbon receptor nuclear translocator 2, ARNT protein 2, Class E basic helix-loop-helix protein 1, bHLHe1, ARNT2, BHLHE1, KIAA0307
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting addition roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively.
Background References:
  • Ramirez, J.M., et al. Eur. J. Immunol. 40(9):2450-2459(2010)
  • Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
  • Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)
  • Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ARNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-278 amino acids from the Central region of human ARNT2.
NCBI Gene ID #:
9915
NCBI Official Name:
Aryl hydrocarbon receptor nuclear translocator 2
NCBI Official Symbol:
ARNT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
79 kDa
Protein Accession #:
Q9HBZ2
Protein GI Number:
125987793
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience
Swissprot #:
Q9HBZ2
User NOte:
Optimal dilutions for each application to be determined by the researcher.